Abstract

Cell fate determination of neural progenitors during brain development is a highly coordinated process influenced by intricate mechanical signals. While mechanical sensing during forebrain development is gaining recognition, the underlying regulatory genes remain underexplored. In this study, we present a comprehensive analysis of the expression patterns of the mechanosensitive channel Tentonin 3 (TMEM150C, TTN3) across critical stages of embryonic brain development (E12.5, E14.5, E16.5, and E18.5) using RNAscope technology. We investigated Ttn3 expression in NSCs and immature neurons through co-staining with Nestin, eomesodermin, and Doublecortin probes. Ttn3 transcripts were detected across multiple embryonic brain regions and layers, with a higher expression in the superficial layers compared to the ventricular (VZ) and subventricular zones (SVZ). Additionally, we demonstrate that TTN3 is involved in the proliferation and migration of neurosphere-derived cells. Our findings suggest that TTN3 may play a critical role in embryonic brain development, potentially contributing as a mechanotransduction mediator.